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unlabeled herring sperm dna  (Promega)

 
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    Promega unlabeled herring sperm dna
    Unlabeled Herring Sperm Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unlabeled+herring+sperm+dna/herring+sperm+dna/pmc05563962-78-19-23
    Average 90 stars, based on 1 article reviews
    unlabeled herring sperm dna - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Alpine soil carbon is vulnerable to rapid microbial decomposition under climate cooling
    Article Snippet: After checking the dye incorporation on a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), DNA was dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45 °C for 45 min. .. Subsequently, labeled DNA was resuspended in 120 μl hybridization solution containing 40% formamide, 3 × SSC, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA), and 0.1% SDS. .. The hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA) according to the manufacturer’s instructions.

    Article Title: Annual Removal of Aboveground Plant Biomass Alters Soil Microbial Responses to Warming
    Article Snippet: .. The labeled DNA was suspended in 120 l hybridization solution containing 50% formamide, 3 SSC (1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 10 g of unlabeled herring sperm DNA (Promega, Madison, WI), and 0.1% SDS. .. After denaturation, hybridizations were performed with a Tecan HS4800 Pro hybridization station (Tecan, USA).

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45°C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95°C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Article Title: Annual Removal of Aboveground Plant Biomass Alters Soil Microbial Responses to Warming
    Article Snippet: .. The labeled DNA was suspended in 120 μl hybridization solution containing 50% formamide, 3× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI), and 0.1% SDS. .. After denaturation, hybridizations were performed with a Tecan HS4800 Pro hybridization station (Tecan, USA).

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers.
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45◦C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95◦C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Hybridization:

    Article Title: Alpine soil carbon is vulnerable to rapid microbial decomposition under climate cooling
    Article Snippet: After checking the dye incorporation on a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), DNA was dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45 °C for 45 min. .. Subsequently, labeled DNA was resuspended in 120 μl hybridization solution containing 40% formamide, 3 × SSC, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA), and 0.1% SDS. .. The hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA) according to the manufacturer’s instructions.

    Article Title: Annual Removal of Aboveground Plant Biomass Alters Soil Microbial Responses to Warming
    Article Snippet: .. The labeled DNA was suspended in 120 l hybridization solution containing 50% formamide, 3 SSC (1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 10 g of unlabeled herring sperm DNA (Promega, Madison, WI), and 0.1% SDS. .. After denaturation, hybridizations were performed with a Tecan HS4800 Pro hybridization station (Tecan, USA).

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45°C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95°C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Article Title: Annual Removal of Aboveground Plant Biomass Alters Soil Microbial Responses to Warming
    Article Snippet: .. The labeled DNA was suspended in 120 μl hybridization solution containing 50% formamide, 3× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI), and 0.1% SDS. .. After denaturation, hybridizations were performed with a Tecan HS4800 Pro hybridization station (Tecan, USA).

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers.
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45◦C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95◦C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    other:

    Article Title: Denitrification and the denitrifier community in coastal microbial mats.
    Article Snippet: The labeled DNAwas resuspended in 50 μl hybridization solution [40% formamide, 5 × SSC, 5 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) and 0.1% SDS] and 2 μl universal standard DNA (0.2 pmol μl−1) labeled with the fluorescent dye Cy-3 (Liang et al., 2010), denatured at 95◦C for 5 min and maintained at 50◦C until loaded onto the microarray slides.

    Article Title: Warming Alters Expressions of Microbial Functional Genes Important to Ecosystem Functioning
    Article Snippet: The Cy5-labeled cDNA was re-suspended in 50 μl hybridization solution [40% formamide, 5x SSC, 5 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA), and 0.1% SDS] and 2 μl universal standard DNA (0.2 pmol μl -1 ) labeled with the fluorescent dye Cy-3 , denatured at 95°C for 3 min and maintained at 65°C until loaded onto microarray slides.

    Article Title: Nitrification and Nitrifying Bacteria in a Coastal Microbial Mat
    Article Snippet: Briefly, 800 ng DNA was labeled with fluorescent Cy-5 dye by random priming and re-suspended in 50 μl hybridization solution (40% formamide, 5 × SSC, 5 μg of unlabeled herring sperm DNA (Promega, Madison, WI), and 0.1% SDS) and 2 μl universal standard DNA (0.2 pmol μl −1 ) labeled with the fluorescent Cy-3 dye (Liang et al., ), denatured for 5 min at 95°C and maintained at 50°C until loaded onto the microarray slides.

    Purification:

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45°C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95°C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers.
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45◦C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95◦C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Incubation:

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45°C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95°C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.

    Article Title: Microbial Community and Functional Structure Significantly Varied among Distinct Types of Paddy Soils But Responded Differently along Gradients of Soil Depth Layers.
    Article Snippet: .. Then the labeled DNA samples were purified with a QIA purification kit (Qiagen, Valencia, CA, USA) and further dried in a SpeedVac (ThermoSavant, Milford, MA, USA) at 45◦C for 45 min. Hybridization buffer containing 40% formamide, 25% SSC, 1% SDS, 10 μg of unlabeled herring sperm DNA (Promega, Madison, WI, USA) was added to the dried DNA samples, then vortexed, spun down and incubated at 95◦C for 5 min. Hybridizations were performed with a MAUI hybridization station (BioMicro, Salt Lake City, UT, USA). .. Subsequently, the microarray was scanned by a 100% laser power and 100% photomultiplier tube with a NimbleGen MS 200 Microarray Scanner (Roche, Madison, WI, USA) and signal intensities were quantified.



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